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The kit is used to detect and quantify the BRAF V600E, E2, D, D2, K, R and M somatic mutations in human genomic DNA. The principle of detection is based on real-time PCR methodology with the use of fluorescently labelled probes and allele-specific primers. The kit contains all components necessary to perform the analysis.
- qPCR assay + Master Mix
- LOD 0.2% mutated BRAF on WT background at 100,000 copies in reaction
- sample concentration 10-80 ng/µl
- positive and negative controls are included
- FAM channel detection
- identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
